Cell Photodamage, a Potential Hazard when Measuring Cytoplasmic Ca2+ with Fura-2.

Resting cytoplasmic free Ca2+ concentration in frog skeletal muscle measured with fura-2 conjugated to high molecular weight dextran

Intact frog skeletal muscle fibers were injected with the Ca2+ indicator fura-2 conjugated to high molecular weight dextran (fura dextran, MW approximately 10,000; dissociation constant for Ca2+, 0.52 microM), and the fluorescence was measured from cytoplasm (17 degrees C). The fluorescence excitation spectrum of fura dextran measured in resting fibers was slightly red-shifted compared with the...

Measurement of [Ca2+]i in whole cell suspensions using fura-2.

Fura-2 measurement of cytosolic free Ca2+ concentration in corpus allatum cells of larval Manduca sexta.

Cytosolic free Ca2+ has been implicated in the regulation of the larval corpus allatum (CA) of the tobacco hornworm Manduca sexta since agents presumed to cause changes in intracellular Ca2+ concentrations affect both basal and neuropeptide-modulated synthesis/release of the juvenile hormones. To determine whether differences in cytosolic free [Ca2+] in CA cells were associated with different l...

Measurement of the matrix free Ca2+ concentration in heart mitochondria by entrapped fura-2 and quin2.

A method was developed to monitor continuously the matrix free Ca2+ concentration ([Ca2+]m) of heart mitochondria by use of the fluorescent Ca2+ indicators, fura-2 and quin2. The acetoxymethyl esters of fura-2 and quin2 were accumulated in and hydrolysed by isolated mitochondria. An increase of the mitochondrial Ca content from 0.3 nmol/mg of protein to 6 nmol/mg corresponded to a rise of [Ca2+...

Measuring Hazard Identification

This paper discusses an experiment that measured the effectiveness of a hazard identification process used to support safety in Defence Standard 00-56 project. The experimental case study utilised a Ministry of Defence project that assessed simultaneously two potential suppliers who were competing for a MOD equipment contract. The UK MOD Corporate Research Programme funded the comparison work a...